Structural basis of Integrator-mediated transcription regulation
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7pks
Q-score: 0.278
Fianu I, Chen Y, Dienemann C, Dybkov O, Linden A, Urlaub H, Cramer P
Science (2021) 374 pp. 883-887 [ DOI: doi:10.1126/science.abk0154 Pubmed: 34762484 ]
- Integrator complex subunit 8 (113 kDa, Protein from Homo sapiens)
- Dsif (Complex from Homo sapiens)
- Rna (Complex from Human immunodeficiency virus 1)
- Integrator complex subunit 6 (100 kDa, Protein from Homo sapiens)
- Serine/threonine-protein phosphatase 2a catalytic subunit alpha isoform (35 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Integrator complex subunit 9 (73 kDa, Protein from Homo sapiens)
- Human integrator-pp2a complex (Complex from Homo sapiens)
- Negative elongation factor c/d (68 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit f (14 kDa, Protein from domestic pig)
- Integrator complex subunit 1 (244 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from domestic pig)
- Non-template dna (14 kDa, DNA from Human immunodeficiency virus 1)
- Dna template (14 kDa, DNA from Human immunodeficiency virus 1)
- Manganese (ii) ion (54 Da, Ligand)
- Unknown (2 kDa, Protein from Homo sapiens)
- Negative elongation factor a (57 kDa, Protein from Homo sapiens)
- Non-template dna (Complex from Human immunodeficiency virus 1)
- Tar rna (5 kDa, RNA from Human immunodeficiency virus 1)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from domestic pig)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from domestic pig)
- Template dna (Complex from Human immunodeficiency virus 1)
- Rna polymerase ii subunit d (16 kDa, Protein from domestic pig)
- Rpbi c-terminal domain peptide (1 kDa, Protein from domestic pig)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Rna polymerase ii subunit k (7 kDa, Protein from domestic pig)
- Integrator complex subunit 4 (108 kDa, Protein from Homo sapiens)
- Integrator complex subunit 5 (108 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase subunit (217 kDa, Protein from domestic pig)
- Serine/threonine-protein phosphatase 2a 65 kda regulatory subunit a alpha isoform (65 kDa, Protein from Homo sapiens)
- Integrator complex subunit 2 (134 kDa, Protein from Homo sapiens)
- Integrator complex subunit 11 (67 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from domestic pig)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from domestic pig)
- Rna_pol_l_2 domain-containing protein (13 kDa, Protein from domestic pig)
- Negative elongation factor e (1 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from domestic pig)
- Pec-integrator-pp2a complex (1 MDa, Complex)
- Nelf (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from domestic pig)
- Pol ii-ec (Complex from Sus scrofa domesticus)
- Negative elongation factor b (68 kDa, Protein from Homo sapiens)
- Integrator complex subunit 7 (106 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b7u
Q-score: 0.483
Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P
Commun Biol (2021) 4 pp. 606-606 [ DOI: doi:10.1038/s42003-021-02088-z Pubmed: 34021257 ]
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from domestic pig)
- Rna_pol_l_2 domain-containing protein (13 kDa, Protein from domestic pig)
- Dna-directed rna polymerase subunit beta (134 kDa, Protein from domestic pig)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from domestic pig)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from domestic pig)
- Uncharacterized protein (7 kDa, Protein from domestic pig)
- Dna-directed rna polymerase ii subunit rpb3 (30 kDa, Protein from domestic pig)
- Putative rna polymerase ii subunit b1 ctd phosphatase rpap2 (69 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Rna polymerase ii subunit k (7 kDa, Protein from domestic pig)
- Dna-directed rna polymerase ii (Complex from Sus scrofa domesticus)
- Rna polymerase ii -rpap2 complex (Complex)
- Dna-directed rna polymerase ii subunit f (14 kDa, Protein from domestic pig)
- Dna-directed rna polymerase subunit (217 kDa, Protein from domestic pig)
- Putative rna polymerase ii subunit b1 ctd phosphatase rpap2 (Complex from Homo sapiens)
- Unknown (2 kDa, Protein from Homo sapiens)